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298 , C1363C1375 (2010)

In vitro transcription to prepare biotinylated PRKAA1 5UTR and scrambled RNA probes PRKAA1 5UTR and scrambled control RNA probes were synthesized using BamHI-linearized pUC57-T7 templates in an 80-l reaction buffer containing 5 transcription buffer (Roche, RPOLT7-RO), 8 l of 10 Dig labeling mixture (Merck, 11277073910), 8 l of 0.1 M DTT (Sigma, D9163