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Following this, the total protein content of each sample was determined through the bicinchoninic acid assay, which was a prerequisite for the subsequent lipid peroxidation assay

Although a direct relationship between high levels of uric acid (UA) and the development of diabetes is still a controversial issue, there is some evidence that strongly points to pancreatic -cells damage as a result of high serum UA levels

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Quantum chemistry analyses have further indicated that structural modifications, particularly electron-donating substituents on luteolin, enhance its MMP-inhibitory activity [129]