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4C), suggesting that 32 23% of the internalized EV-derived-Rac1 were released to the cytosol under the assumption of the observation rate of the Halo tag to be 100% (Supplementary Fig

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The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4C overnight on a rotating shaker