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If needed, TB-500 can be continued to be taken at 1mg/daily until achieving full recovery among test subjects

For proliferation analysis, expanded HBs were fixed with 4% PFA, incubated in blocking and permeabilizing buffers (0.1% Triton X-100, 5% normal donkey serum), and then incubated with APC-conjugated Ki67 and other indicated antibodies [1]

24 , e13626 (2023)