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In addition, subcellular structures such as mitochondria, vacuoles, and endoplasmic reticulum as well as reactive oxygen species (ROS) accumulation were observed under a fluorescence microscopy after stained with fluorescent dyes such as 27-DCF diacetate, Mito Tracker Green FM, Vacuole Membrane Marker MDY-64, and ER-Tracker Red dye

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Taken together, our findings suggest an attenuation, but not a blocking, of ketamines acute antidepressant effects with naltrexone pretreatment in a sample of patients with MDD