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In summary, Methylene Blue is contraindicated in pregnancy due to clear evidence of fetal risk (intestinal atresia, fetal death, etc.), and it should only be considered in life-threatening maternal situations when no safer alternative exists

The interaction features a total of 23 hydrogen bonds and eight salt bridges distributed over separate interaction surfaces on domain IV (DIV) and DI of the adjacent protomer, resulting in 1,260 2 of buried surface area on gB (Fig