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For leaner individuals, 45 degrees ensures the needle reaches the fat layer without penetrating muscle

Forward and reverse primer pairs for qRT-PCR were purchased from Bioneer, and the primer sequences are listed in Tables S1 and S2

Frssing S, Nylander M, Chabanova E, Frystyk J, Holst JJ, Kistorp C, et al

However, recent modelling suggests that an EAT Lancet diet achieved by 2050 alongside reductions in FLW and projected per capita incomes rising faster than food prices, could be less expensive globally on average than BAU projected diets 58 and, in a similar vein, we find that (producer) prices would be lower relative to BAU projections