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Transfection, BSO treatment, and exposure of AQ-QI and DEAQ-QI were identical as described in section Transient Transfection of Human GSTP1 Gene and Cell Viability Assay. After exposure the medium was gently removed and the later steps were conducted according to manufacturers manual

Cells become corneocytes, the outer protective shield that prevents water loss and blocks environmental damage

Another issue is the concomitant generation of Fe 2+ , a known prooxidant that can produce additional radicals, such as OH from H 2 O 2