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Comparative example 4 Arg34GLP-1 (9-37) with a modification of Lys at position 26: adding N, N-diisopropylethylamine into 300mL of water mixed solution, cooling to 0-5 , adjusting the pH to 11.5, adding 1g of Arg34GLP-1 (9-37) to form a mixture, dissolving 0.33g of the compound 1 into 100mL of acetonitrile, dropwise adding the mixture into the mixture, reacting for 3 hours at 0-5 , removing the acetonitrile by rotary evaporation, diluting the obtained concentrated solution with water to a certain concentration, freeze-drying the water phase, and obtaining the compound 2 with the purity of only 78.52%, wherein a specific liquid chromatogram is shown in figure 18

There are real reasons for the variation

Severe or persistent side effects warrant discontinuation and medical evaluation

Injecting a frozen or thawed semaglutide pen may cause injection site reactions, reduced efficacy, or unpredictable glucose effects