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Specifically, RBX2 binds Cul5 via its N-terminus and engages ubiquitination E2 enzymes (UBE2C and UBE2S) through its RING domain, thereby acting as a ubiquitination E3 ligase that catalyzes the transfer of ubiquitin from the E2 to substrates, predominantly assembling polyubiquitin chains via K11 linkages This interaction enables direct ubiquitination of targeted substrates through a thioester intermediate on the E2 enzyme (33, 34, 41)

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The most common are nausea, bloating, tiredness, constipation, and diarrhoea

[3] This discovery opened the door to an entire class of diabetes treatments