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Aging (Reduced Synthesis Capacity) As you age, production of glutathione declines due to: Reduced activity of gamma-glutamylcysteine ligase (GCL) , the rate-limiting enzyme in glutathione synthesis Decreased cellular energy (ATP) to fuel glutathione recycling Lower levels of precursor amino acids (especially cysteine and glycine) Studies show glutathione levels drop 10-15% per decade after age 40 [3]

These data collectively suggest that the inhibitory effect of GSSG on the NF-B signaling pathway, via suppression of IKK- activation, is mechanistically implicated in the increased sensitivity of hepatocytes to TNF killing

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