While GHK-Cu is generally well-tolerated in topical research, certain contraindications or precautions should be considered, especially for systemic research or in specific populations: Systemic Research: For systemic applications, GHK-Cu is generally not recommended for subjects who are pregnant, breastfeeding, have active cancer, or suffer from Wilsons disease (a condition involving copper accumulation)
The study used specific concentrations in topical applications: PGD2 at 10 micrograms, PTD-DBM at 10 millimolar, DHT at 100 micromolar, and KY19382 at 2 millimolar
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2016 measured 55.8% wrinkle volume reduction and 32.8% depth reduction over 12 weeks, with a 31.6% greater reduction than Matrixyl 3000 in head-to-head testing