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Log-phase Mtb cultures were subjected to nutrient starvation in Middlebrook 7H9 broth lacking albumin, dextrose, and sodium chloride (ADS) or any additional carbon source, and supplemented with tyloxapol, a non-metabolisable detergent, to prevent bacterial clumping
However, the dysbiosis caused by microplastics reduces the indole-producing microbiota, leading to insufficient tryptophan metabolites and disrupting this immune regulatory mechanism [145]