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Protein was eluted with 10 mM glutathione in 50 mM Tris, pH 8.0, 5% glycerol, and examined for purity by SDS-PAGE and Coomassie staining

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168 The 2020 KDOQI guidelines for the management of kidney diseases recommends that people with stage 35 CKD who are not receiving dialysis eat a low-protein diet (0.550.60 grams per kilogram of body weight per day [g/kg/day]) or a very-low-protein diet (0.280.43 g/kg/day) supplemented with 715 grams of keto-analogues of essential amino acids

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