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Reconstitution Process: Allow the BPC-157 vial to reach room temperature Use bacteriostatic water (BAC water) as the reconstitution fluid (this contains 0.9% benzyl alcohol as a preservative) Draw the appropriate amount of BAC water into an insulin syringe Inject the water slowly down the inside wall of the vial, allowing it to gently dissolve the powder Do not shake vigorously, but gentle swirling is acceptable Allow the solution to sit until fully dissolved (typically a few minutes) Common Reconstitution Ratio: 5 mg BPC-157 + 5 mL BAC water = 1 mg/mL (100 mcg per 0.1 mL / 10 units on an insulin syringe) Storage Guidelines: Lyophilized (unreconstituted) BPC-157: Store below -18C (-0.4F) for long-term storage

The DAC version requires 1,000 to 2,000 mcg once or twice weekly

Injection site

The pellet was then resuspended in 1X PBS (pH 7.4), centrifuged (500 x g, 5 min), and aspirated