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Peptide separation was performed using a Dionex Ultimate 3000 UPLC system (Thermo Fisher Scientific) and a reversed-phase XBridge BEH C18 column (3.5 m, 3.0 150mm, Waters Corporation) stepped gradient and Fractions were then collected and analysed on Orbitrap Lumos Tribrid mass spectrometer equipped with the FAIMS Pro ion mobility system interfaced with nLC 1200 liquid chromatography system (Thermo Fisher Scientific)

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