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Quantification of ATM expression Cultured hair follicle melanocytes were seeded into 8-well chamber slides (~ 10,000 cells/well) and left to attach for at least 24 h, fixed in ice-cold methanol for 10 min, and incubated with blocking buffer of 10% donkey serum (Sigma-Aldrich, UK) for at least 30 min

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Proteins were obtained by centrifugation at 12000 rpm for 10 min and were detected by a BCA protein assay kit (CWBIO, CW0014S)