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the lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C

Potential Side Effects of Early Dosing When you take a dose early, you are essentially increasing the amount of medication in your body sooner than your system expects

This suggests that reduced BSA is responsible for the apparent extra GST activity detected in our samples