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Conclusion and Future Perspectives Current research shows that MO exerts its multiple immune-related effects primarily through directly eliminating pathogens or modulating the balance of pro- and anti-inflammatory mediators released from various kinds of immune cells by regulating the activity of signaling pathways, such as the canonical NF-B pathway (Figure 1)

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After 24 h, migrated cells on the upper surface of the filter were removed with a cotton swab, while the cells on the bottom of the filter were fixed with 4% paraformaldehyde at room temperature for 15 min and stained with 3% crystal violet solution for 15 min