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In studies on Achilles tendon tendinopathy in mice, it was observed that aged tendons exhibited elevated expression of inflammationrelated biomarkers, including inflammatory disulfide HMGB1, macrophage marker CD68, and senescenceassociated markers such as SAgal, p53, and p16 [63]

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Some data suggests that injecting before 8am may produce slightly lower post-prandial glucose levels compared to evening injections, with one analysis showing a 15% improvement in post-meal blood sugar management